era
168
GTP-binding protein, involved in the assembly of the 30S ribosomal subunit
locus
BSU_25290
Molecular weight
33.92 kDa
pI
8.38
function
ribosome assembly
product
GTP-binding protein
essential
yes
synonyms
era, bex, yqfH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1159 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,610,041 → 2,610,946
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
Protein family
[wiki|TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily] (according to UniProt)
[wiki|Domains]
Era-type G domain (aa 7-174) (according to UniProt)
KH type-2 domain (aa 205-282) (according to UniProt)
Structure
[PDB|3IEV] ([protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era] from ''Aquifex aeolicus'', 50% identity/ 77% similarity, complex with the 3' end of [wiki|16S rRNA]) [Pubmed|19706445]
[AF|P42182]
Effectors of protein activity
binds ppGpp [Pubmed|26951678], ppGpp may inhibit activity [Pubmed|26951678]
Paralogous protein(s)
[protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]
[wiki|Localization]
cytoplasm, throughout the cell [pubmed|36406443]
Expression and Regulation
Operons
genes
[gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]-[gene|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era]-[gene|33FBC31E8C24177137B0BDD8B46C1AFFD7F7B038|yqzL]-[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]
description
[Pubmed|22383849]
expression
expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|6330116], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|6330116], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP837, available in [wiki|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP845, available in [wiki|Stülke] lab
labs
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
Original Publications
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Time of last update: 2024-10-15 01:07:55
Author of last update: Jstuelk