alr
168
alanine racemase
locus
BSU_04640
Molecular weight
43.10 kDa
pI
8.43
function
peptidoglycan biosynthesis
product
alanine racemase
essential
yes
ec
5.1.1.1
synonyms
alr, dal, alrA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0787 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
517,372 → 518,541
Phenotypes of a mutant
reported to be essential [Pubmed|12682299], however deletion mutants have been reported by Ferrari et al. (1985) and Xia et al (2007) [Pubmed|17602222]
auxotrophy for D-alanine
a [gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP] [gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr] double mutant is not viable due to the inability to acquire D-alanine [pubmed|33155333]
the mutant is not viable in liquid culture but it can live in a mixed biofilm (with wild type) due to D-ala sharing [pubmed|33594973]
The protein
Catalyzed reaction/ biological activity
L-alanine --> D-alanine (according to UniProt)
Protein family
alanine racemase family (with [protein|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD], according to UniProt)
Structure
[PDB|1L6G] (with N-[5�-phosphopyridoxyl]-D-alanine, Geobacillus stearothermophilus), [PDB|1SFT] (Geobacillus stearothermophilus)
[AF|P10725]
Paralogous protein(s)
[protein|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]
Expression and Regulation
Operons
genes
[gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]-[gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
description
[pubmed|22383849]
Biological materials
Mutant
[http://www.ncbi.nlm.nih.gov/nuccore/402479429?report=genbank&from=517383&to=518551 alr-1], two-base mutation 526AG>T (=R176X), creating a frameshift and an immediate stop codon, available in [http://bgsc.org/ BGSC] 1A4, a D-alanine auxotroph
References
Ferrari E, Henner DJ, Yang MY. Isolation of an Alanine Racemase Gene from Bacillus subtilis and its Use for Plasmid Maintenance in B. subtilis, Nature Biotechnol 1985.Nov;3:1003-7.
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Time of last update: 2024-12-21 02:03:05
Author of last update: Jstuelk