yngH
168
methylcrotonoyl-CoA carboxylase
locus
BSU_18240
Molecular weight
48.92 kDa
pI
7.31
function
mother cell metabolism, leucine utilization
product
methylcrotonoyl-CoA carboxylase, subunit
essential
no
ec
6.4.1.4
synonyms
yngH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4770 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,953,181 → 1,954,515
The protein
Catalyzed reaction/ biological activity
ATP + hydrogencarbonate + N6-biotinyl-L-lysyl-[protein] --> ADP + H+ + N6-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
acetyl-CoA + ATP + hydrogencarbonate --> ADP + H+ + malonyl-CoA + phosphate (according to UniProt)
[wiki|Domains]
[wiki|ATP-grasp domain] (aa 120-317) (according to UniProt)
Biotin carboxylation domain (aa 1-444) (according to UniProt)
[wiki|Cofactors]
biotin
Structure
[PDB|1ULZ] (biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus, 52% identity) [Pubmed|14993673]
[AF|O34544]
Expression and Regulation
Operons
genes
[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
description
[Pubmed|12662922]
regulation
expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-B086 (yngH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1085 NBRP B. subtilis, Japan]
BKE18240 (Δ[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACTTTTGTAAACATGATTT, downstream forward: _UP4_CACCTATAAAGGAGGCAGTT
BKK18240 (Δ[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACTTTTGTAAACATGATTT, downstream forward: _UP4_CACCTATAAAGGAGGCAGTT
References
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Time of last update: 2024-12-19 19:15:18
Author of last update: Melvin.boenninger