ssdC
168
MucB/RseB-like protein, affects the assembly of the spore cortex and spore shape
locus
BSU_04630
Molecular weight
42.03 kDa
pI
9.6
function
assembly of the spore cortex
product
spore shape determinant C
essential
no
synonyms
ydcC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2834 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
516,241 → 517,257
Phenotypes of a mutant
defect in sporulation [Pubmed|14523133]
some production of small spores, reduced [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activity [Pubmed|26735940]
forespores are rounder than those of the wild type [pubmed|33315869]
produces spores with an abnormal-looking cortex, abolishing cortex synthesis in the [gene|CA71F577FDBB8C6E647AA56E16E4111651F47E17|ssdC] mutant largely supresses the spore shape defect [pubmed|33315869]
The protein
[wiki|Domains]
N-terminal transmembrane segment and a large extracellular C-terminal-domain [pubmed|33315869]
Structure
[AF|P96619]
Modification
phosphorylated on Thr-197 and Thr-201 [Pubmed|20509597]
[wiki|Localization]
cell membrane [pubmed|33315869]
forms a discontinuous, dynamic ring-like structure in the peripheral membrane of the mother cell, near the mother cell proximal pole of the forespore [pubmed|33315869]
localization depends on [protein|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055|spoVM], [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA], [protein|6698BB092E03BEF48AFD0CBF565410109CD1ABB5|spoVID], and [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA] [pubmed|33315869]
Expression and Regulation
Operons
genes
[gene|CA71F577FDBB8C6E647AA56E16E4111651F47E17|ssdC]
description
[pubmed|22383849]
regulation
expressed in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|15699190,12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-C122 (ydcC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2120 NBRP B. subtilis, Japan]
BKE04630 (Δ[gene|CA71F577FDBB8C6E647AA56E16E4111651F47E17|ssdC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACCCCTTTTTCTCAATTG, downstream forward: _UP4_TAACCGCCAAAGGCCAAACA
BKK04630 (Δ[gene|CA71F577FDBB8C6E647AA56E16E4111651F47E17|ssdC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATGATAACCTCCTTT, downstream forward: _UP4_TAGTCTGCATATTAGGGAAA
References
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Time of last update: 2024-12-22 00:26:42
Author of last update: Jstuelk