ydjI
168
required for membrane localization of [protein|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA] (together with [protein|F93381205C61665820D034A384BD60410AAE84C1|ydjG] and [protein|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH])
locus
BSU_06210
Molecular weight
35.92 kDa
pI
4.94
function
targeting of [protein|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]
product
unknown
essential
no
ec
null
synonyms
ydjI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4260 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
673,814 → 674,785
Phenotypes of a mutant
reduced membrane fluidity [pubmed|34777311]
The protein
Catalyzed reaction/ biological activity
membrane localization of [protein|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA] (together with [protein|F93381205C61665820D034A384BD60410AAE84C1|ydjG] and [protein|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH]) [pubmed|34777311]
Protein family
Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) domain protein family (with [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA] and [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT])
[wiki|Domains]
contains a SPFH domain (Stomatin, Prohibitin, Flotillin, and HflK/C-Domain) (aa 22 - 232) [pubmed|34777311]
C-terminal zinc-finger domain (aa 300 - 322) [pubmed|34777311]
Structure
[AF|O34789]
[wiki|Localization]
cell membrane (fluid regions) (via [protein|F93381205C61665820D034A384BD60410AAE84C1|ydjG] and [protein|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH])) [pubmed|34777311]
Expression and Regulation
Operons
genes
[gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]-[gene|F93381205C61665820D034A384BD60410AAE84C1|ydjG]-[gene|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH]-[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]
description
[Pubmed|11454200]
regulation
induced by alkaline shock ([protein|search|SigW]) [Pubmed|11454200]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18840696,20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11454200], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
MGNA-C216 (ydjI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2214 NBRP B. subtilis, Japan]
BKE06210 (Δ[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGCCTCTCCCTTTCT, downstream forward: _UP4_TAAGAATCATACAGTGAAAA
BKK06210 (Δ[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGCCTCTCCCTTTCT, downstream forward: _UP4_TAAGAATCATACAGTGAAAA
References
Page visits: 3876
Time of last update: 2025-03-14 22:36:21
Author of last update: Jstuelk