mcsA
168
regulatory subunit of the [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] arginine kinase holoenzyme, modulator of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-dependent repression, [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA] activates kinase activity of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]
locus
BSU_00840
Molecular weight
20.88 kDa
pI
6.62
function
control of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR] activity
product
activator of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] kinase activity
essential
no
synonyms
mcsA, yacH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3880 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
101,927 → 102,484
The protein
Catalyzed reaction/ biological activity
binding to and activation of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] arginine kinase activity [pubmed|38625935,39232187]
[wiki|Domains]
[wiki|UVR domain] (aa 139-174) (according to UniProt)
Structure
[PDB|8WTC] [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] kinase domain complexed with [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA] [pubmed|38625935]
[PDB|8GQD] the [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA]4-[protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]4 complex of S. aureus [pubmed|39232187]
[AF|P37569]
Modification
phosphorylated on Arg-169 [Pubmed|22517742]
Expression and Regulation
Operons
genes
[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
description
[Pubmed|8793870]
regulation
expressed during germination and spore outgrowth [Pubmed|24244006]
regulatory mechanism
[protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|protein:908DB17A39D518E84977250C55825E77FA02E391|ctsR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|30962353], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8793870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
MGNA-B929 (yacH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1928 NBRP B. subtilis, Japan]
''mcsA::aphA3'' available from the Gerth lab
BKE00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT, downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
BKK00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT, downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
Antibody
available in Gerth lab
References
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Time of last update: 2024-12-19 00:59:15
Author of last update: Jstuelk