Explore the novel features of the upcoming release of in its beta version,
SubtiWiki v5! It features genomic conservation, interaction of proteins with metabolites, dedicated metabolite pages, and much more!
Check the pages for
MntR and
manganese!
mcsA
168
regulatory subunit of the [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] arginine kinase holoenzyme, modulator of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-dependent repression, [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA] activates kinase activity of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]
Molecular weight
20.88 kDa
function
control of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR] activity
product
activator of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] kinase activity
Genomic Context
This gene is a member of the following
regulons
Gene
Coordinates
101,927 → 102,484
The protein
Catalyzed reaction/ biological activity
binding to and activation of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] arginine kinase activity [pubmed|38625935,39232187]
[wiki|Domains]
[wiki|UVR domain] (aa 139-174) (according to UniProt)
Structure
[PDB|8WTC] [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] kinase domain complexed with [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA] [pubmed|38625935]
[PDB|8GQD] the [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA]4-[protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]4 complex of S. aureus [pubmed|39232187]
[AF|P37569]
Modification
phosphorylated on Arg-169 [Pubmed|22517742]
Expression and Regulation
Operons
genes
[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
description
[Pubmed|8793870]
regulation
expressed during germination and spore outgrowth [Pubmed|24244006]
regulatory mechanism
[protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|protein:908DB17A39D518E84977250C55825E77FA02E391|ctsR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|30962353], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8793870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Open in new tab
[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]→[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
2024-10-09 06:18:32
Bzhu
132
b7066b5ca5004b095502d71420c469e9ee925bfa
163769A1708F45F8ABE642374A97FA8A00293529
Biological materials
Mutant
MGNA-B929 (yacH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1928 NBRP B. subtilis, Japan]
''mcsA::aphA3'' available from the Gerth lab
BKE00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT, downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
BKK00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT, downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
Antibody
References
12923084,8793870,11179229,9987115,16497325,19226326,9987115,11544224,22517742,36815589,38625935,39232187