dnaD
168
initiation of chromosome replication, part of the [wiki|replisome]
locus
BSU_22350
Molecular weight
27.49 kDa
pI
7.17
function
[category|SW.3.1.1|DNA replication]
product
initiation of chromosome replication
essential
yes
synonyms
dnaD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3935 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,345,433 → 2,346,131
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds a ssDNA motif in oriC [pubmed|37093985]
[protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth] endonuclease activity at AP sites [Pubmed|21954439]
required for the assembly of the replicative helicase [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC] at the origin of replication [Pubmed|22821970]
interaction with [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] inhibits the ability of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] to bind cooperatively to DNA [Pubmed|22821970]
Structure
[PDB|2C79] (N-terminal domain)
[AF|P39787]
[wiki|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]-[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]-[gene|4B2A124B726393544A47791E5A5681F7B766A588|ypoC]
description
[Pubmed|22383849]
genes
[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]
description
[Pubmed|21954439]
regulation
constitutively expressed [Pubmed|21954439]
labs
[wiki|Alan Grossman], MIT, Cambridge, MA, USA
References
Reviews
Original Publications
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Time of last update: 2024-12-21 02:32:09
Author of last update: Jstuelk