spoIIP
168
cell wall hydrolase with both amidase and endopeptidase activities, required for dissolution of the septal cell wall
locus
BSU_25530
Molecular weight
44.39 kDa
pI
6.85
product
cell wall hydrolase
essential
no
synonyms
spoIIP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5819 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,633,237 → 2,634,442
The protein
Catalyzed reaction/ biological activity
removes the stem peptides from the cell wall and cleaves the cross-links between them [Pubmed|20159959]
required for proper localization of [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] (together with [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]) [Pubmed|23834622]
Structure
[AF|P37968]
Effectors of protein activity
activity is stimulated by interaction with [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID] [Pubmed|20159959]
[wiki|Localization]
cell membrane [Pubmed|20159959]
the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]
localization of SpoIIP to the leading edge depends on peptidoglycan synthesis at the leading edge of the engulfing membrane [Pubmed|27852437]
localization of the [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]-[protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]-[protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] complex to the septal membrane depends on [protein|D98D61CA651AAA415386B1B2B41562E5CF77DEF1|spoIIB] [pubmed|36066101]
Expression and Regulation
Operons
genes
[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]-[gene|3F3695BA670B48D19256F7229A8F89BFADF41038|yqxA]
description
[Pubmed|16497325]
regulation
expressed early during sporulation ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE],[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325,7836306]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|7836306], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
genes
[gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]-[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]
description
[Pubmed|16497325]
regulation
expressed during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF], [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], [wiki|SpoVT]) [Pubmed|16497325,1840582]
regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|16497325], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325,1840582], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|1840582], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
additional information
[protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
BKE25530 (Δ[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAATCCCTCCAGCGCT, downstream forward: _UP4_AAACAATAAGAAATAGGTGG
BKK25530 (Δ[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAATCCCTCCAGCGCT, downstream forward: _UP4_AAACAATAAGAAATAGGTGG
References
Reviews
Original Publications
Page visits: 3107
Time of last update: 2024-12-30 15:58:13
Author of last update: Jstuelk