rluD
168
pseudouridylate synthase, required for modification of 23S rRNA at positions 1940, 1944, and 1946
locus
BSU_15460
Molecular weight
33.50 kDa
pI
5.43
function
23S rRNA modification
product
pseudouridylate synthase
essential
no
ec
5.4.99.23
synonyms
ylyB, ylmL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,617,210 → 1,618,121
The protein
Catalyzed reaction/ biological activity
uridine in helix 69 of 23S rRNA (positions 1940, 1944, 1946) --> pseudouridine in RNA [pubmed|39036956]
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO] and [protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT], according to UniProt)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 15-74) (according to UniProt)
Structure
[PDB|1V9F] (RluD from E. coli, 42% identical) [pubmed|15078091]
[AF|Q45480]
Paralogous protein(s)
[protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT], [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
Expression and Regulation
Operons
genes
[gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT, downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
BKK15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT, downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
References
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Time of last update: 2024-12-21 13:00:28
Author of last update: Jstuelk