rluD

rluD
168

pseudouridylate synthase, required for modification of 23S rRNA at positions 1940, 1944, and 1946

locus
BSU_15460
Molecular weight
33.50 kDa
pI
5.43
Protein length
Gene length
function
23S rRNA modification
product
pseudouridylate synthase
essential
no
ec
5.4.99.23
synonyms
ylyB, ylmL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,617,210 → 1,618,121
The protein
Catalyzed reaction/ biological activity
uridine in helix 69 of 23S rRNA (positions 1940, 1944, 1946) --> pseudouridine in RNA [pubmed|39036956]
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO] and [protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT], according to UniProt)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 15-74) (according to UniProt)
Structure
[PDB|1V9F] (RluD from E. coli, 42% identical) [pubmed|15078091]
[AF|Q45480]
Paralogous protein(s)
[protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT], [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
Expression and Regulation
Operons
genes
[gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
description
[Pubmed|22383849]
Open in new tab

[gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]→[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]

2024-09-28 16:35:21

Jstuelk

66

8de7ab701af29d04cf1775c3714df5244d59efd2

D260A981278938463A8A0903EAC8DF3268BD4008

Biological materials
Mutant
BKE15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT,  downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
BKK15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT,  downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
References
17188032,15078091,39036956

C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495

Page visits: 1749

Time of last update: 2024-10-01 07:34:41

Author of last update: Jstuelk