pgm
168
[metabolite|phosphoglycerate] mutase, glycolytic / gluconeogenic enzyme
locus
BSU_33910
Molecular weight
56.14 kDa
pI
5.21
function
enzyme in glycolysis / gluconeogenesis
product
2,3-bisphosphoglycerate-independent [metabolite|phosphoglycerate] mutase
essential
Yes
ec
5.4.2.1
synonyms
pgm, gpmI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0696 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,477,877 3,479,412
Phenotypes of a mutant
Essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
(2R)-[metabolite|2-phosphoglycerate] --> 3-[metabolite|phosphoglycerate] (according to UniProt)
Protein family
BPG-independent [metabolite|phosphoglycerate] mutase family (single member, according to UniProt)
[wiki|Cofactors]
Mn2+
Structure
[PDB|1EJJ] (Geobacillus stearothermophilus, complex with 3-[metabolite|phosphoglycerate]), [PDB|1EQJ] (Geobacillus stearothermophilus, complex with [metabolite|2-phosphoglycerate]), ''Geobacillus stearothermophilus'', complex with [metabolite|2-phosphoglycerate] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=16359 NCBI], ''Geobacillus stearothermophilus'', complex with 3-[metabolite|phosphoglycerate] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=15578 NCBI]
[AF|P39773]
Modification
phosphorylation on Ser-62 [Pubmed|17218307], [Pubmed|17726680]
Effectors of protein activity
Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione [Pubmed|33963]
2,3-Diphosphoglycerate has NO role on this enzyme regulation [Pubmed|33963]
Kinetic information
Reversible Michaelis-Menten [Pubmed|33963]
[wiki|Localization]
Cytoplasm (Homogeneous) [Pubmed|16479537]
Additional information
extensive information on the structure and enzymatic properties of Pgm can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglycerate_Mutase Proteopedia]
Expression and Regulation
Operons
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
regulation
expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
Biological materials
Mutant
GP593 (''pgm''::''cat''), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
GP598 (''pgm''::''erm''), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
GP698 (''pgm''-''eno''::cat), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
Expression vectors
pGP1425 (expression of ''pgm'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jrg Stlke]'s lab
pGP1500 (expression of ''pgm'' and ''eno'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jrg Stlke]'s lab
pGP1101 (N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jrg Stlke]'s lab
pGP396 (Pgm-S62A, N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jrg Stlke]'s lab
pGP92 (N-terminal Strep-tag, for [wiki|SPINE], expression in B. subtilis, in [wiki|pGP380]), available in [wiki|Jrg Stlke]'s lab, [pubmed|19193632]
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab, [pubmed|19193632]
labs
[wiki|Jrg Stlke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[wiki|Mark J. Jedrzejas], Research Center Oakland, CA, USA [http://www.chori.org/Principal_Investigators/Jedrzejas_Mark_J/jedrzejas_research.html Homepage]
References
Page visits: 8565
Time of last update: 2024-12-20 13:29:04
Author of last update: Jstuelk