phoR
168
two-component sensor kinase, regulation of phosphate metabolism
locus
BSU_29100
Molecular weight
64.95 kDa
pI
5.96
function
regulation of phosphate metabolism
product
two-component sensor kinase
essential
no
synonyms
phoR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5002 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,976,068 → 2,977,807
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments
[wiki|PAS domain], for binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate)) [Pubmed|25315493]
[wiki|Histidine kinase domain] (aa 357-575) (according to UniProt)
Structure
[PDB|3CWF] (extracellular domain) [Pubmed|20008068]
[AF|P23545]
Modification
autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [wiki|PAS domain] of [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR] [Pubmed|25315493]
Effectors of protein activity
activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate) to the intracellular [wiki|PAS domain] of [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR] [Pubmed|25315493]
the kinase activity is activated by an intermediate in the WTA biosynthetic pathway (product of [protein|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA] or [protein|911A4D957A6C0DFC636A152395119DBFC777DDA2|tagB]) [pubmed|29644746]
Paralogous protein(s)
[protein|E88978809272FAC2520DB4ABCA0554F8028F3451|walK], [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]
description
[Pubmed|14762014]
regulation
carbon catabolite repression ([protein|search|CcpA]) [Pubmed|16452408,12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|16452408,12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|20382764], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, due to [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]~P binding to the 3’end of the [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR] operon [Pubmed|25666134,15205429,14762014], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16452408], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15205429], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,15205429], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
1A966 ( ''phoR''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
BKE29100 (Δ[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA
BKK29100 (Δ[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA
labs
[wiki|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-17 10:55:57
Author of last update: Jstuelk