pcrA
168
ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer, required to overcome a replicative stress
locus
BSU_06610
Molecular weight
83.37 kDa
pI
5.77
function
coupling of DNA replication, transcription, recombination and segregation
product
ATP-dependent DNA helicase
essential
yes
synonyms
pcrA, yerF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0210 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
719,370 → 721,589
Phenotypes of a mutant
essential [Pubmed|12682299]
inactivation can be suppressed by inactivation of either [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA], [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ], [gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO], or [gene|AD1F39823ABCE2A222259B879513752942C59590|mfd] [pubmed|32793628]
inactivation can be partially suppressed by inactivation of [gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [pubmed|33920686]
The protein
Catalyzed reaction/ biological activity
coupling of DNA replication, transcription, recombination and segregation [pubmed|32793628]
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
dismantles [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] filaments [Pubmed|20723756]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
preferentially unwinds ssDNA in the 3′→ 5′ direction [pubmed|33920686]
unwinds DNA-RNA hybrids [pubmed|33920686]
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|Domains]
two RecA-like domains [Pubmed|24147116]
intrinxically disordered C-terminal domain (for interaction with [wiki|RNA polymerase]) [Pubmed|24147116]
[wiki|UvrD-like helicase ATP-binding domain](aa 10-289) (according to UniProt)
[wiki|UvrD-like helicase C-terminal] (aa 290-570) (according to UniProt)
Structure
[PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
[PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
[PDB|5DMA], C-terminal [wiki|RNA polymerase] interaction domain [pubmed|28160601]
[AF|O34580]
Effectors of protein activity
unwinding of the double-stranded ICEBs1 DNA is stimulated by [protein|search|HelP] [Pubmed|23326247]
helicase activity is stimulated by interaction with [wiki|RNA polymerase] [Pubmed|24147116]
Paralogous protein(s)
[protein|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|yjcD]
Expression and Regulation
Operons
genes
[gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
description
[Pubmed|9701819]
regulation
induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
Page visits: 5055
Time of last update: 2024-12-20 19:31:47
Author of last update: Jstuelk