fin
168
[wiki|RNA polymerase]-binding protein, feedback inhibitor of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF] activity
locus
BSU_00540
Molecular weight
8.71 kDa
pI
5.19
function
control of [wiki|sporulation]
product
feedback inhibitor of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF] activity
essential
no
synonyms
fin, subA, yabK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5867 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
60,130 → 60,360
Phenotypes of a mutant
sporulation defect (50-fold reduction of viable spore count) and arrest after engulfment [Pubmed|21037003]
The protein
Catalyzed reaction/ biological activity
binding of Fin to the [protein|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|ß'] subunit of [wiki|RNA polymerase] prevents binding of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF], and thus [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]-dependent transcription [pubmed|28598017]
Structure
[PDB|5MSL] [pubmed|28598017]
[AF|P37553]
[wiki|Localization]
localizes diffusely within the forespore compartment of sporangia at both early and late stages of sporulation, cytosolic protein [Pubmed|21037003]
Expression and Regulation
Operons
genes
[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]
description
[pubmed|22383849]
regulation
expressed in two waves in the forespore (after two hours ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) and three hours ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]) after the onset of sporulation) [Pubmed|21037003]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|21037003], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|21037003], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
genes
[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]-[gene|FE7859E98F26E532AE02CD71B42B845BADBCF06E|pth]-[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]
description
[pubmed|22383849]
regulation
induced under stress conditions ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [pubmed|15805528]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
genes
[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]-[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]-[gene|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]
description
[pubmed|22383849]
expression
expression is heterogeneous [pubmed|35171018]
Biological materials
Mutant
MGNA-B913 (yabK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1912 NBRP B. subtilis, Japan]
BKE00540 (Δ[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGGGTCCTCCTGAT, downstream forward: _UP4_TAAAAAGCTTTGGTGTAGAC
BKK00540 (Δ[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGGGTCCTCCTGAT, downstream forward: _UP4_TAAAAAGCTTTGGTGTAGAC
References
Reviews
Original Publications
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Time of last update: 2025-03-18 09:30:55
Author of last update: Jstuelk