trmK

trmK
168

tRNA:m1A22 methyl transferase

locus
BSU_25180
Molecular weight
23.56 kDa
pI
5.27
Protein length
Gene length
function
tRNA modification
product
tRNA:m1A22 methyl transferase
essential
no
synonyms
trmK, yqfN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2384 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,598,616 → 2,599,266
The protein
Catalyzed reaction/ biological activity
Catalyzes the S-adenosyl-L-methionine-dependent formation of N1-methyladenine at position 22 (m1A22) in tRNA [pubmed|18420655]
adenosine22 in tRNA + S-adenosyl-L-methionine --> H+ + N1-methyladenosine22 in tRNA + S-adenosyl-L-homocysteine [pubmed|18420655]
Protein family
TrmK family (single member, according to UniProt)
[wiki|Domains]
N-terminal Class I MTase domain [pubmed|30931478]
C-terminal coiled-coil domain [pubmed|30931478]
[wiki|Cofactors]
SAM [pubmed|30931478]
Structure
[PDB|6Q56] [pubmed|30931478]
[AF|P54471]
Modification
phosphorylation on Ser-48 [Pubmed|17218307]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]
description
[pubmed|22383849]
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[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]

2024-12-02 17:35:57

Bzhu

82

9ec9c207745419570f1f0961ed03149c30ec6d94

9F3F3DD68AF9DBDEA3CD7785AC2CFD3A317F0543

Biological materials
Mutant
BKE25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA,  downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
BKK25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA,  downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
References
18420655,17218307,27098549,30931478,38331425

C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85

Page visits: 2889

Time of last update: 2024-11-29 17:38:42

Author of last update: Jstuelk