trmK
168
tRNA:m1A22 methyl transferase
locus
BSU_25180
Molecular weight
23.56 kDa
pI
5.27
function
tRNA modification
product
tRNA:m1A22 methyl transferase
essential
no
synonyms
trmK, yqfN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2384 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,598,616 → 2,599,266
The protein
Catalyzed reaction/ biological activity
Catalyzes the S-adenosyl-L-methionine-dependent formation of N1-methyladenine at position 22 (m1A22) in tRNA [pubmed|18420655]
adenosine22 in tRNA + S-adenosyl-L-methionine --> H+ + N1-methyladenosine22 in tRNA + S-adenosyl-L-homocysteine [pubmed|18420655]
Protein family
TrmK family (single member, according to UniProt)
[wiki|Domains]
N-terminal Class I MTase domain [pubmed|30931478]
C-terminal coiled-coil domain [pubmed|30931478]
[wiki|Cofactors]
SAM [pubmed|30931478]
Structure
[PDB|6Q56] [pubmed|30931478]
[AF|P54471]
Modification
phosphorylation on Ser-48 [Pubmed|17218307]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]
description
[pubmed|22383849]
Biological materials
Mutant
BKE25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
BKK25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
References
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Time of last update: 2024-11-29 17:38:42
Author of last update: Jstuelk