cotQ
168
outer spore coat protein
locus
BSU_34520
Molecular weight
49.92 kDa
pI
6.1
function
protection of the spore
product
spore coat protein
essential
no
synonyms
cotQ, yvdP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0277 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,542,943 → 3,544,286
The protein
Protein family
oxygen-dependent FAD-linked oxidoreductase family (with [protein|23F2385D14DDD5C400DC897E0F0FC814ACFC36B8|yitY] and [protein|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK], according to UniProt)
[wiki|Domains]
[wiki|FAD-binding PCMH-type domain] (aa 29-201) (according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|3W8W] (EncM from Streptomyces maritimus, 32% identity) [pubmed|24162851]
[AF|O06997]
Paralogous protein(s)
[protein|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK], (40%)
[wiki|Localization]
outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]
Expression and Regulation
Operons
genes
[gene|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ]
description
[Pubmed|12480901]
regulation
expressed late during sporulation ([protein|search|SigG], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,15699190,12480901]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|12480901], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-B620 (yvdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1619 NBRP B. subtilis, Japan]
BKE34520 (Δ[gene|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATTATTTCACTCCCA, downstream forward: _UP4_TAAAGTTTATTAAAGACAGA
BKK34520 (Δ[gene|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATTATTTCACTCCCA, downstream forward: _UP4_TAAAGTTTATTAAAGACAGA
References
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Time of last update: 2024-12-21 14:51:41
Author of last update: Melvin.boenninger