acoC
168
acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
locus
BSU_08080
Molecular weight
42.73 kDa
pI
6.52
function
acetoin utilization
product
acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
essential
no
ec
2.3.1.12
synonyms
acoC, yfjI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0508 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
881,049 → 882,245
The protein
Catalyzed reaction/ biological activity
(R)-N6-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA --> (R)-N6-(S8-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA (according to UniProt)
Protein family
[wiki|2-oxoacid dehydrogenase family] (according to UniProt)
[wiki|Domains]
[wiki|Lipoyl-binding domain] (aa 2-77) (according to UniProt)
[wiki|Peripheral subunit-binding domain] (PSBD) (aa 118-155) (according to UniProt)
[wiki|Cofactors]
lipoic acid
Structure
[PDB|3DUF] (PDH from Geobacillus stearothermophilus, 33% identity) [pubmed|19081062]
[AF|O31550]
Paralogous protein(s)
[protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB], [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB], [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]
[wiki|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]-[gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]-[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]-[gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]
description
[pubmed|11274109]
regulation
induced by acetoin ([protein|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]: activation, (interaction with [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]-containing [wiki|RNA polymerase]) [ PubMed|11274109], in [regulon|protein:706864AAF36684AD5E46F30E9EB76315AB412700|acoR regulon]
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: indirect positive regulation, in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [PubMed|11274109], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|21815947]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
MGNA-C282 (acoC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2280 NBRP B. subtilis, Japan]
BKE08080 (Δ[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC, downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC
BKK08080 (Δ[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC, downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC
labs
[wiki|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-10-11 23:48:31
Author of last update: Jstuelk