opuBC
168
choline and arsenocholine [wiki|ABC transporter] (binding protein)
locus
BSU_33710
Molecular weight
34.25 kDa
pI
6.62
function
compatible solute transport
product
choline and arsenocholine [wiki|ABC transporter] (binding protein)
essential
no
synonyms
opuBC, proX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1732 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,460,503 → 3,461,423
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine [pubmed|29159878]
Protein family
OsmX family (with [protein|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC], according to UniProt)
Structure
[PDB|3R6U], [PDB|6EYQ] (in complex with choline) [pubmed|21658392]
[PDB|5NXX] (in complex with arsenocholine) [pubmed|29159878]
[PDB|6EYL] (in complex with carnitine)
[PDB|6EYG] (mutant in complex with betaine)
[PDB|6EYH] (mutant in complex with dimethylsulfoniopropionate)
[AF|Q45462]
Paralogous protein(s)
[protein|CBA6108A10AD8B932BC5B19A0E7982D0F56F1E08|opuCC]
[wiki|Localization]
associated to the membrane (via [protein|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[protein|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]) [Pubmed|10092453]
lipoprotein [Pubmed|10216873]
Expression and Regulation
Operons
genes
[gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
description
[Pubmed|10216873]
regulation
induced by choline ([protein|search|GbsR]) [Pubmed|22408163]
regulatory mechanism
[protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|protein:5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR regulon]
[protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|protein:9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR regulon]
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10216873], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An antisense RNA is predicted for'[protein|search|opuBD]' [PubMed|20525796]
Biological materials
Mutant
BKE33710 (Δ[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCTTTTCATGAGCCGCC, downstream forward: _UP4_GAATCGTGAAAGGGGGAAGA
BKK33710 (Δ[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCTTTTCATGAGCCGCC, downstream forward: _UP4_GAATCGTGAAAGGGGGAAGA
References
Reviews
Original Publications
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Time of last update: 2024-12-20 09:41:02
Author of last update: Jstuelk