dgcK
168
diguanylate cyclase
locus
BSU_09120
Molecular weight
40.53 kDa
pI
8.57
function
synthesis of c-di-GMP
product
diguanylate cyclase
essential
no
synonyms
dgcK, yhcK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2199 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
985,734 → 986,813
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-GMP from two molecules of GTP [Pubmed|23893111]
[wiki|Domains]
six transmembrane helices at the N-terminus (according to UniProt?)
contains a C-terminal [wiki|GGDEF domain] (aa 223-357) [Pubmed|22821967]
Structure
[PDB|2WB4] (the C-terminal [wiki|GGDEF domain], PleD from Caulobacter vibrioides, 44% identity)
[AF|P54595]
[wiki|Localization]
cell membrane at cell poles and septa, probably close to [protein|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK] [pubmed|28536559]
present at a single site in the cell membrane [pubmed|32156823]
Expression and Regulation
Operons
genes
[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-A656 (yhcK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/656 NBRP B. subtilis, Japan]
GP848 (''[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]''::''ermC''), available in [wiki|Jörg Stülke]'s lab [pubmed|28420751]
BKE09120 (Δ[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATATTATCACCCTTATA, downstream forward: _UP4_TGAATTCAATGTTCGAATTC
BKK09120 (Δ[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATATTATCACCCTTATA, downstream forward: _UP4_TGAATTCAATGTTCGAATTC
References
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Time of last update: 2024-12-21 11:33:28
Author of last update: Jstuelk