melE
168
subunit of [wiki|ABC transporter] for melibiose and raffinose (binding lipoprotein)
locus
BSU_30270
Molecular weight
48.07 kDa
pI
5.85
function
uptake of melibiose and raffinose
product
[wiki|ABC transporter] (binding protein)
essential
no
synonyms
melE, msmE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,097,850 → 3,099,130
The protein
Catalyzed reaction/ biological activity
binding of melibiose and raffinose [pubmed|31138628]
Protein family
[wiki|Bacterial solute-binding protein 1 family] (according to UniProt)
Structure
[PDB|4R6H]
[AF|O34335]
[wiki|Localization]
extracellular (signal peptide), associated to the cell membrane (via [protein|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[protein|05BFEA711D82395A55B505E5A38286BE2F570350|melD]) [Pubmed|18957862,10092453]
Expression and Regulation
Operons
genes
[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]
description
[Pubmed|22383849]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
genes
[gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]-[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]
description
[Pubmed|22383849]
regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]: repression, [pubmed|31138628], in [regulon|protein:58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22900538,31138628], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A801 (msmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/801 NBRP B. subtilis, Japan]
BKE30270 (Δ[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT, downstream forward: _UP4_TCCGAAACACAGGGAGCTGA
BKK30270 (Δ[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT, downstream forward: _UP4_TCCGAAACACAGGGAGCTGA
References
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Time of last update: 2024-12-22 08:19:41
Author of last update: Jstuelk