ycgT

ycgT
168

NADPH:ferredoxin oxidoreductase

locus
BSU_03270
Molecular weight
36.81 kDa
pI
5.61
Protein length
Gene length
function
unknown
product
NADPH:ferredoxin oxidoreductase
essential
no
synonyms
ycgT

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0492 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
352,858 → 353,868
The protein
Catalyzed reaction/ biological activity
H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
Protein family
ferredoxin--NADP reductase type 2 family (with [protein|BAA74A6971CED9FB55540F1BC602CDA287D83E74|yumC], according to UniProt)
[wiki|Cofactors]
FAD [Pubmed|25826316]
Structure
[PDB|5XHU]
[AF|O31475]
Paralogous protein(s)
[protein|BAA74A6971CED9FB55540F1BC602CDA287D83E74|yumC]
Additional information
The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
Expression and Regulation
Operons
genes
[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]
description
[pubmed|22383849]
regulation
induced under conditions of iron starvation ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|16672620]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [Pubmed|16672620], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
additional information
the mRNA is very stable (half-life > 15 min) [Pubmed|12884008]
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[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]

2024-11-09 11:19:04

Jstuelk

72

051e8ac5689d8158f132195c7420e821fb6b8f43

A7049AD5890F7B0CC6E9E3A7AB7C2DB2BDBF17C4

Biological materials
Mutant
MGNA-C052 (ycgT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2050 NBRP B. subtilis, Japan]
GP1852 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::cat), available in [wiki|Jörg Stülke]'s lab
BKE03270 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT,  downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
BKK03270 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT,  downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
References
12884008,16672620,25826316,20878669

C35A302F0B0BC724EC91B47AB4D38CF56CADD574

Page visits: 3880

Time of last update: 2024-12-24 12:09:09

Author of last update: Melvin.boenninger