minJ
168
topological determinant of [wiki|cell division], part of the Min system (with Z ring placement)
locus
BSU_35220
Molecular weight
43.51 kDa
pI
9.06
function
[wiki|cell division] site selection
product
topological determinant of [wiki|cell division]
essential
no
synonyms
minJ, yvjD, swrAB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0265 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,620,346 → 3,621,539
Phenotypes of a mutant
formation of minicells [pubmed|33849976]
increased cell length [pubmed|33849976,34846166]]
inactivation of [gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ] reduces sporulation efficiency to 36% that of wild type cells; delayed entry into sporulation and aberrant forespores [Pubmed|26735940]
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle [Pubmed|20352045]
required for oriC placement during spore development [Pubmed|27059541]
Protein family
MinJ family (single member, according to UniProt)
[wiki|Domains]
7 transmembrane helices (aa 15- 269) (according to UniProt)
[wiki|PDZ domain] (aa 295 - 361) (according to UniProt)
Structure
[AF|O34375]
[wiki|Localization]
forms rings at the division septum [Pubmed|22108385]
cell membrane [Pubmed|23264578]
Additional information
about 580 molecules per cell [pubmed|33849976]
Expression and Regulation
Operons
genes
[gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]-[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
description
[Pubmed|16030230]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, ([protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] promoter) [Pubmed|18567663], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18567663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|18567663], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
genes
[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
description
[pubmed|18976281]
Biological materials
Mutant
MGNA-B648 (minJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1647 NBRP B. subtilis, Japan]
BKE35220 (Δ[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTATCTCTCACCGCCTCA, downstream forward: _UP4_TAAAAGGCAGCCCGGCACCG
BKK35220 (Δ[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTATCTCTCACCGCCTCA, downstream forward: _UP4_TAAAAGGCAGCCCGGCACCG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]
[wiki|Marc Bramkamp], University of Cologne, Cologne, Germany [http://www.kraemerlab.uni-koeln.de/celldivision.php homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 10:13:14
Author of last update: Jstuelk