fpbQ
168
petrobactin (3.4-catecholate siderophore) [wiki|ABC transporter] (binding protein), major component of the secretome
locus
BSU_03830
Molecular weight
34.64 kDa
pI
6.63
function
[wiki|acquisition of iron]
product
petrobactin [wiki|ABC transporter] (binding protein)
essential
no
synonyms
fpbQ, yclQ, fpiA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4607 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
435,036 → 435,989
The protein
Catalyzed reaction/ biological activity
uptake of the siderophore petrobactin [Pubmed|19955416]
Protein family
[wiki|Bacterial solute-binding protein 8 family] (according to UniProt)
[wiki|Domains]
[wiki|Fe/B12 periplasmic-binding domain] (aa 56-317) (according to UniProt)
Structure
[PDB|3GFV]
[AF|P94421]
[wiki|Localization]
associated to the membrane (via [protein|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[protein|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]) [Pubmed|10092453,18763711]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]
description
[Pubmed|19955416]
regulation
immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-C067 (yclQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2065 NBRP B. subtilis, Japan]
BKE03830 (Δ[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA, downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC
BKK03830 (Δ[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA, downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC
References
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Time of last update: 2024-10-12 14:36:19
Author of last update: Melvin.boenninger