yitS

yitS
168

fatty acid binding protein

locus
BSU_11110
Molecular weight
31.12 kDa
pI
5.63
Protein length
Gene length
function
phosphorylation of fatty acids
product
fatty acid binding protein
essential
no
synonyms
yitS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1307 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,187,700 → 1,188,551
The protein
Catalyzed reaction/ biological activity
binds and presents fatty acids for phosphorylation to [protein|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA] (based on findings for homologous proteins from Staphylococcus aureus) [pubmed|30429218]
[wiki|Domains]
DegV domain (aa 3-282) (according to UniProt)
Structure
[PDB|1PZX] (Geobacillus stearothermophilus)
[AF|P70945]
Paralogous protein(s)
[protein|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV]
Expression and Regulation
Operons
genes
[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]
description
[Pubmed|22383849]
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[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]

2024-10-26 20:40:11

Jstuelk

63

812504db80ea64f4355e7f50b0d765470f5e248f

9F51780C04B3A95B15B52D0F3CD52618F287D277

Biological materials
Mutant
GP3756 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::aphA3), available in [wiki|Jörg Stülke]'s lab
MGNA-B196 (yitS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1195 NBRP B. subtilis, Japan]
BKE11110 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC,  downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
BKK11110 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC,  downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
Expression vectors
pGP3626 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMOadapt), available in [wiki|Jörg Stülke]'s lab
References
Research papers
30429218

C13F1870B014F8B2C16D9B169F40747DE5B51B85

Page visits: 2586

Time of last update: 2024-11-03 18:38:52

Author of last update: Dennis.wicke