yitS
168
fatty acid binding protein
locus
BSU_11110
Molecular weight
31.12 kDa
pI
5.63
function
phosphorylation of fatty acids
product
fatty acid binding protein
essential
no
synonyms
yitS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1307 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,187,700 → 1,188,551
The protein
Catalyzed reaction/ biological activity
binds and presents fatty acids for phosphorylation to [protein|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA] (based on findings for homologous proteins from Staphylococcus aureus) [pubmed|30429218]
[wiki|Domains]
DegV domain (aa 3-282) (according to UniProt)
Structure
[PDB|1PZX] (Geobacillus stearothermophilus)
[AF|P70945]
Paralogous protein(s)
[protein|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV]
Expression and Regulation
Operons
genes
[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]
description
[Pubmed|22383849]
Biological materials
Mutant
GP3756 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::aphA3), available in [wiki|Jörg Stülke]'s lab
MGNA-B196 (yitS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1195 NBRP B. subtilis, Japan]
BKE11110 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
BKK11110 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
Expression vectors
pGP3626 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMOadapt), available in [wiki|Jörg Stülke]'s lab
References
Research papers
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Time of last update: 2024-11-03 18:38:52
Author of last update: Dennis.wicke