citM
168
Mg2+-citrate transporter
locus
BSU_07610
Molecular weight
45.67 kDa
pI
6.23
function
uptake of citrate and magnesium
product
Mg2+-citrate transporter
essential
no
synonyms
citM, yflO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2851 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
834,383 → 835,684
Phenotypes of a mutant
no growth with citrate as sole carbon source [Pubmed|10972810]
The protein
Protein family
[wiki|CitM transporter family (TC 2.A.11)] (according to UniProt)
Structure
[AF|P55069]
Paralogous protein(s)
[protein|71A73C825981E74842FC28D1B05CF85E9F3F5A56|citH]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]-[gene|66B9809A77AB15E5C8C8E5A8E26752771AF55E3A|yflN]
description
[Pubmed|10972810]
regulation
induced by citrate ([protein|search|CitT]) [Pubmed|10972810]
regulatory mechanism
[protein|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]: activation, [Pubmed|10972810], in [regulon|protein:1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538,10972810], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10972810], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C252 (citM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2250 NBRP B. subtilis, Japan]
GP2244 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''ermC''), available in [wiki|Jörg Stülke]'s lab
GP2760 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan''), available in [wiki|Jörg Stülke]'s lab
GP2761 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''spec''), available in [wiki|Jörg Stülke]'s lab
BKE07610 (Δ[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATTCCCCCTTAAA, downstream forward: _UP4_TGATAATTCAGGCGTAATGA
BKK07610 (Δ[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATTCCCCCTTAAA, downstream forward: _UP4_TGATAATTCAGGCGTAATGA
GP2763 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan'' ''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP2767 ''citM-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP2965 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-10-15 11:17:28
Author of last update: LKrueger