cydA
168
cytochrome bd ubiquinol oxidase (subunit I), high affinity terminal oxidase
locus
BSU_38760
Molecular weight
52.13 kDa
pI
8.94
function
respiration
product
cytochrome bd ubiquinol oxidase (subunit I), high affinity terminal oxidase
essential
no
synonyms
cydA, yxkK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1271 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,977,791 → 3,979,197
The protein
Catalyzed reaction/ biological activity
2 ubiquinol + n H+ + O2 --> 2 ubiquinone + n H+ + 2 H2O (according to UniProt)
Protein family
cytochrome ubiquinol oxidase subunit 1 family (with [protein|EB8AF295A11F28BDFAE39038FA89890824FC7791|ythA], according to UniProt)
[wiki|Cofactors]
heme b, heme d (according to UniProt)
Structure
[PDB|5DOQ] (from Geobacillus thermodenitrificans, 30% identity) [pubmed|27126043]
[AF|P94364]
Paralogous protein(s)
[protein|EB8AF295A11F28BDFAE39038FA89890824FC7791|ythA]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA]-[gene|E66EAB3C172ED8D1D26C675A7338B8153FB66987|cydB]-[gene|A65576E0261F1950A0DAD72B2432D97AB3EB2AA5|cydC]-[gene|244BF9E397FDB0691FFAA8C9D419B0AE417CACE1|cydD]
description
[Pubmed|16207915]
regulation
expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]) [Pubmed|17322317]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]: repression, [Pubmed|15231791], in [regulon|protein:B5EF521437323EF43F08E5EFDB5C798616CA499A|rex regulon]
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|17322317], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [PubMed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
MGNA-B745 (cydA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1744 NBRP B. subtilis, Japan]
BKE38760 (Δ[gene|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTTCTCCTCCATTTC, downstream forward: _UP4_GATCCATTTAGTCAGGAGGT
BKK38760 (Δ[gene|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTTCTCCTCCATTTC, downstream forward: _UP4_GATCCATTTAGTCAGGAGGT
lacZ fusion
pGP3879 (kan, based on [wiki|pAC7]]), available in [wiki|Jörg Stülke]'s lab
GP4302 ([gene|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA]-lacZ::aphA3), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-20 01:46:04
Author of last update: Dennis.wicke