yetL
168
transcriptional repressor of [gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM] and [gene|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|yetL], induction in response to flavonoids
locus
BSU_07220
Molecular weight
19.07 kDa
pI
7.52
function
regulation of [gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM] expression
product
transcription repressor ([wiki|MarR family])
essential
no
synonyms
yetL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
789,652 → 790,155
Phenotypes of a mutant
a point mutation in yetL may be involved in the acquisition of resistance to L-[metabolite|asparagine] [pubmed|38193659]
The protein
Protein family
[wiki|MarR family]
[wiki|Domains]
[wiki|HTH marR-type domain] (aa 26-160) (according to UniProt)
Structure
[PDB|7WZE] [pubmed|35367827]
[AF|O31541]
Effectors of protein activity
inducers: flavonoids of kaempferol, apigenin, and luteolin [Pubmed|19329649]
Expression and Regulation
Operons
genes
[gene|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|yetL]
description
[Pubmed|19329649]
Biological materials
Mutant
MGNA-B463 (yetL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1462 NBRP B. subtilis, Japan]
BKE07220 (Δ[gene|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|yetL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGCAATTAGCTCCCGTA, downstream forward: _UP4_TAAAAAACCCGTCCGATCCG
BKK07220 (Δ[gene|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|yetL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGCAATTAGCTCCCGTA, downstream forward: _UP4_TAAAAAACCCGTCCGATCCG
Expression vectors
pGP3934 (Cc-terminal Strep-tag, purification from E. coli, in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-21 07:19:23
Author of last update: Dennis.wicke