tasA
168
major component of biofilm matrix, forms bundles of fibres
locus
BSU_24620
Molecular weight
28.15 kDa
pI
5.44
function
[wiki|biofilm formation]
product
major component of biofilm matrix
essential
no
synonyms
tasA, cotN, yqhF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5884 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,553,081 → 2,553,866
Phenotypes of a mutant
altered cell death pattern in colonies [Pubmed|23012477]
increases membrane fluidity and cell death [pubmed|32313019]
failure to produce structured and wrinkled colonies [pubmed|33803642]
The protein
Catalyzed reaction/ biological activity
forms amyloid fibers that bind cells together in the biofilm [Pubmed|20080671]
Protein family
peptidase M73 family (single member, according to UniProt)
Structure
[PDB|5OF1] [pubmed|29531041]
[PDB|5OF2] [pubmed|29531041]
[AF|P54507]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862], secretion requires [protein|09B082BF39703F0D9A5980E643175DBD59F5B228|sipW] [Pubmed|10049401]
Expression and Regulation
Operons
genes
[gene|8C13E1EF5437DD8F9906643DEDCBB18DABE3B9E3|tapA]-[gene|09B082BF39703F0D9A5980E643175DBD59F5B228|sipW]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]
description
[Pubmed|16430695]
regulation
repressed by casamino acids [Pubmed|12107147]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [PubMed|10464223,17720793], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|16430695], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
[protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR]: activation, [Pubmed|24196425], in [regulon|protein:E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16430695], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI] or [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|slrA] [PubMed|15661000,19788541] or by SlrR [PubMed|20351052]
expression of the operon is localized to a ring near the periphery of the biofilm [pubmed|29590605]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-C449 (yqhF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2447 NBRP B. subtilis, Japan]
BKE24620 (Δ[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA, downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
BKK24620 (Δ[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA, downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
References
Reviews
Original Publications
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Time of last update: 2024-12-06 00:28:16
Author of last update: Jstuelk