msrB
168
peptide methionine sulfoxide reductase
locus
BSU_21680
Molecular weight
16.46 kDa
pI
5.84
function
regeneration of methionine and restoration of protein function after oxidative damage
product
peptide methionine sulfoxide reductase
ec
1.8.4.12
synonyms
msrB, yppQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0229 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,287,097 → 2,287,528
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + H2O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein] (according to UniProt)
Protein family
MsrB Met sulfoxide reductase family (single member, according to UniProt)
[wiki|Domains]
MsrB domain (aa 5-126) (according to UniProt)
Structure
[PDB|1XM0]
[AF|P54155]
Expression and Regulation
Operons
genes
[gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]
description
[Pubmed|18662407]
regulation
induced by oxidative stress ([protein|search|Spx])
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, oxidized [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] binds [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA] and enhances [protein|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[protein|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB] operon transcription [Pubmed|18662407], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18662407,17289755], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A905 (yppQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/905 NBRP B. subtilis, Japan]
BKE21680 (Δ[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTTCTTTATTGTACG, downstream forward: _UP4_TAAACTGTTAAAAACAGGCC
BKK21680 (Δ[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTTCTTTATTGTACG, downstream forward: _UP4_TAAACTGTTAAAAACAGGCC
References
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Time of last update: 2024-10-14 10:01:05
Author of last update: Melvin.boenninger