rhgR
168
transcriptional activator ([wiki|AraC family]) of the rhamnogalacturonan operon
locus
BSU_07010
Molecular weight
87.56 kDa
pI
7.77
function
regulation of pectin utilization
product
transcriptional activator of the rhamnogalacturonan operon
essential
no
synonyms
rhgR, yesS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2207 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
765,838 → 768,123
The protein
Protein family
[wiki|AraC family]
[wiki|Domains]
[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]-binding domain: aa 406 - 510 [Pubmed|19651770]
[wiki|HTH araC/xylS-type domain] (aa 659-756) (according to UniProt)
Structure
[AF|O31522]
Effectors of protein activity
interaction with [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr(His)-P]]] (occurs in the absence of preferred carbon sources) stimulates YesS activity [Pubmed|19651770]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|A96A83684E1E6E8705265D3932DA60FF47C23E3B|rhiL]-[gene|2DC415DCC2ECC3B46526108638DDB46C28832041|rhiF]-[gene|0B11FACEB5E96D166AC1EA0E32C68FCDAF8327E4|rhiG]-[gene|8EED04FCE6E2900B729178209FB281F5463B2540|rhiN]-[gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
description
[Pubmed|22383849]
regulation
induced by pectin ([protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR], [protein|F09661C8A483D0FC796204102021104A4373F895|rhgL]) [Pubmed|19651770,35881471]
repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [pubmed|35881471]
regulatory mechanism
[protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]: activation, [Pubmed|19651770], in [regulon|protein:BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: Sigma factor, [pubmed|35881471], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|F09661C8A483D0FC796204102021104A4373F895|rhgL]: activation, [pubmed|35881471], in [regulon|protein:F09661C8A483D0FC796204102021104A4373F895|rhgL regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|35881471], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Biological materials
Mutant
MGNA-B450 (yesS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1449 NBRP B. subtilis, Japan]
BKE07010 (Δ[gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTCTCCCCCCGCCGC, downstream forward: _UP4_GAAACGCCGTGAAAGGAGAG
BKK07010 (Δ[gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTCTCCCCCCGCCGC, downstream forward: _UP4_GAAACGCCGTGAAAGGAGAG
References
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Time of last update: 2024-12-31 05:19:43
Author of last update: Jstuelk