rocR

rocR
168

transcriptional activator of arginine utilization operons

locus
BSU_40350
Molecular weight
52.01 kDa
pI
6.08
Protein length
Gene length
function
transcriptional activator of arginine utilization operons
product
transcriptional activator
essential
no
synonyms
rocR

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3829 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,145,747 → 4,147,132
Phenotypes of a mutant
''rocR'' is unable to use arginine as the sole nitrogen source, indicating that RocR is a positive regulator of arginine catabolism. [pubmed|8113162]
The protein
Catalyzed reaction/ biological activity
has ATPase activity: ATP + H2O --> ADP + H+ + phosphate [pubmed|37343703]
transcription activation at [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]-dependent promoters of [gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocABC], [gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocDEF], and [gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [pubmed|37343703]
Protein family
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93| Sigma-54] interacting transcription activator
[wiki|Transcription factors activating transcription at SigL-dependent promoters]
[wiki|Domains]
N-terminal [wiki|PAS domain] (aa 10-125) for effector (ornithine) binding [pubmed|37343703]
central [wiki|Sigma-54 factor interaction domain] and AAA+ ATPase domain (aa 143-372) [pubmed|37343703]
2x nucleotid-binding domain (171–178),(233–242)
HTH motif (434–453)
[wiki|Cofactors]
ornithine is required for ATPase activity and RocR-dependent transcription activation [pubmed|37343703]
Structure
[PDB|1OJL] (from Salmonella typhimurium, corresponds to aa 143 ... 454, 41% identity) [pubmed|16005641]
[AF|P38022]
Effectors of protein activity
ornithine is required for ATPase activity and RocR-dependent transcription activation [pubmed|37343703]
ATP binding and hydrolysis are required for the formation of the transcriptionally active hexamers [pubmed|37343703]
Expression and Regulation
Operons
genes
[gene|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]
description
[pubmed|22383849]
regulation
autoregulation by [protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 7540694 PubMed]
regulatory mechanism
[protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]: autorepression, [Pubmed|2548995], in [regulon|protein:BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR regulon]
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: autorepression, in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [PubMed|7540694], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]

2024-08-09 16:55:03

Jstuelk

59

da0ea8a191fd183accf76cfb073c603795d527cb

D542BE061E47614773C854ADD156C7F4703B8C41

Biological materials
Mutant
GP2312 (Δ[gene|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]::''tet'', available in [wiki|Jörg Stülke]'s lab)
QB5533 (aphA3), from [wiki|Michel Debarbouille], available in [wiki|Jörg Stülke]'s lab
1A911 ( ''rocR''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A911&Search=1A911 BGSC]
BKE40350 (Δ[gene|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE40350 (Δ[gene|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGTATGAACCTCCCTCA,  downstream forward: _UP4_CAATATCGGCTGAAGAAATT
BKK40350 (Δ[gene|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGTATGAACCTCCCTCA,  downstream forward: _UP4_CAATATCGGCTGAAGAAATT
Expression vectors
pGP3855: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab [Pubmed|37343703]
pGP3856 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP4091 ''rocR-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
References
Reviews
34160574
Original Publications
12634342,8113162,9194709,21965396,23869754,21906631,16005641,37343703

BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA

Page visits: 5736

Time of last update: 2024-10-15 00:35:08

Author of last update: Jstuelk