mpfA
168
primary magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation
locus
BSU_09550
Molecular weight
49.69 kDa
pI
4.4
function
export of magnesium
product
magnesium efflux pump
essential
no
synonyms
mpfA, yhdP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,032,063 → 1,033,397
Phenotypes of a mutant
suppression of the growth defects of [gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA] and [gene|5F3933197343FBBDCBD42B408837F8EEF0A0BEBD|rplW] mutants [pubmed|29967120]
partial suppression of sporulation frequency of a [gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA] mutant [pubmed|29967120]
suppresses the Mn2+ sensitivity of a [gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR] [gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] double mutant [pubmed|31964700]
increased tolerance to Mn2+ and Co2+ [pubmed|31964700]
resistant to high Mg2+ concentrations [pubmed|31964700]
significantly delayed osmoadaptation to both high NaCl and high KCl [pubmed|35164567]
The protein
Protein family
[wiki|UPF0053 family](according to UniProt)
[wiki|Domains]
[wiki|CNNM transmembrane domain] (aa 1-201) (according to UniProt)
[wiki|DUF21 domain] (aa 5-201)
2 [wiki|CBS domain]s (aa 220-282, aa 284-344) (according to UniProt)
Structure
[PDB|4HG0] (CorC from E. coli, corresponds to the [wiki|CBS domain]s and the C-terminal transporter-associated domain)
[AF|O07585]
Paralogous protein(s)
[protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB], [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA], [protein|A459410312A926365B45CEB4694DE399B38820A2|yugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|yhdT]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|EDFAF3AF6F2E7578733D5BF0295AD614070453F4|yhdQ]-[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]
description
[Pubmed|22383849]
Biological materials
Mutant
HB24928 (Δ[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]), available in [wiki|John Helmann]'s and [wiki|Jörg Stülke]'s labs
MGNA-A696 (yhdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/696 NBRP B. subtilis, Japan]
BKE09550 (Δ[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
BKK09550 (Δ[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
Expression vectors
pGP2931 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3463 (214-349aa) (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3468 (214-349aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-21 15:27:26
Author of last update: Jstuelk