yfhO
168
required for lipoteichoic acid glycosylation
locus
BSU_08610
Molecular weight
93.56 kDa
pI
9.63
function
lipoteichoic acid glycosylation
product
glycosyltransferase
essential
no
synonyms
yfhO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4485 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
931,879 → 934,464
Phenotypes of a mutant
expression of [protein|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB] in the absence of [protein|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO] causes constitutive activation of [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] [Pubmed|23632331]
The protein
Catalyzed reaction/ biological activity
moves GlcNAc sugar moieties from a C55-P intermediate onto the lipoteichoic acid backbone on the outside of the cell [pubmed|29343515]
[wiki|Domains]
13 predicted transmembrane helices and a large extracellular loop of 389 amino acids connecting the last two transmembrane helices [pubmed|29343515]
Structure
[AF|O31582]
[wiki|Localization]
cell membrane, with large extracellular loop [pubmed|29343515]
Expression and Regulation
Operons
genes
[gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]-[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]
description
[Pubmed|22383849,8921856]
regulation
induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|8921856,15805528,9636707]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|8921856,9636707], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|9636707], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
Biological materials
Mutant
MGNA-C320 (yfhO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2318 NBRP B. subtilis, Japan]
BKE08610 (Δ[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAATTCTCCAGTCTT, downstream forward: _UP4_CTCGTCAGTCTGCTGCTTGC
BKK08610 (Δ[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAATTCTCCAGTCTT, downstream forward: _UP4_CTCGTCAGTCTGCTGCTTGC
References
Page visits: 2581
Time of last update: 2024-12-21 03:24:15
Author of last update: Jstuelk