yxiD
168
LXG toxin, non-specific metal-dependent RNase
locus
BSU_39300
Molecular weight
64.13 kDa
pI
9.4
function
competetion with other bacteria in biofilms
product
LXG toxin, RNase
essential
no
ec
null
synonyms
yxiD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,036,784 → 4,038,493
Phenotypes of a mutant
the mutant is outcompeted by wild type cells in biofilms [pubmed|34280190]
The protein
[wiki|Domains]
[wiki|LXG domain] (aa 1-235) (according to UniProt)
Structure
[AF|P42296]
[wiki|Localization]
secreted
secretion and delivery requires [protein|50D2A03E2D7B5D461540FD157377E0D37F771888|WXG100] and the T7SS ([protein|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[protein|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[wiki|YukB]-[wiki|YueB]-[wiki|YueC]-[protein|B258CA26B0EC2310475FCCD67F1F196857FC0004|yueD]) [pubmed|34280190]
Expression and Regulation
Operons
genes
[gene|75B0239578C92D4F3B298CBB077635BF5567C590|yxiB]-[gene|F44437883F39E5240D36340A7DD07803C5090ABB|yxiC]-[gene|BD8C0563C7123B6DEC1EEB602BDF2C9D92D34991|yxiD]-[gene|0DAFBDDC5E41592D06F97D404E75AF14B6783729|yxxD]-[gene|2710D9FD5FA50E09B4E482C13140F69B13C71861|yxxE]
description
[Pubmed|22383849,7883710,10746760]
Biological materials
Mutant
MGNA-B717 (yxiD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1716 NBRP B. subtilis, Japan]
BKE39300 (Δ[gene|BD8C0563C7123B6DEC1EEB602BDF2C9D92D34991|yxiD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCCCTTGTCCTCCCGCG, downstream forward: _UP4_AAATGAGTTATAATTTTATA
BKK39300 (Δ[gene|BD8C0563C7123B6DEC1EEB602BDF2C9D92D34991|yxiD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCCCTTGTCCTCCCGCG, downstream forward: _UP4_AAATGAGTTATAATTTTATA
References
Page visits: 4112
Time of last update: 2024-12-20 10:41:11
Author of last update: Jstuelk