ylmH
168
ribosome-associated quality control factor, stabilizes the P-site tRNA conformation, processivity factor essential for poly-alanine synthesis during ribosome quality control elongation by [protein|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH], redundant with [protein|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP]
locus
BSU_15410
Molecular weight
28.92 kDa
pI
7.04
function
ribosome-associated quality control
product
ribosome-associated quality control factor
essential
no
synonyms
ylmH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2302 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,611,654 → 1,612,427
The protein
Catalyzed reaction/ biological activity
binds to the 50S ribosome subunit via 23S rRNA [pubmed|38811035]
binds peptidyl-tRNA-50S complexes in a position analogous to that of S4-domain-containing protein [protein|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP] [pubmed|38811035]
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 181-251) [pubmed|38811035]
Structure
[PDB|8S1P] [protein|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH] bound to the 50S ribosome subunit [pubmed|38811035]
[PDB|8S1U] [protein|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH] and [protein|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH] bound to the 50S ribosome subunit [pubmed|38811035]
[PDB|2FPH] (from Streptococcus pneumoniae, N-terminal domain, corresponds to aa 4 ... 158, 32% identity)
[AF|P71020]
Expression and Regulation
Operons
genes
[gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]-[gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]-[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]-[gene|2B2D285D5A8F160808A21DCF07BDB924365970C5|ylmG]-[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]
description
[Pubmed|16420366]
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
Biological materials
Mutant
MGNA-B364 ([gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1363 NBRP B. subtilis, Japan]
BKE15410 (Δ[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTCTTTCCCTTCTATG, downstream forward: _UP4_TAGTCGGTTTTTCAGTTTTT
BKK15410 (Δ[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTCTTTCCCTTCTATG, downstream forward: _UP4_TAGTCGGTTTTTCAGTTTTT
References
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Time of last update: 2024-12-21 22:32:13
Author of last update: Jstuelk