rpoC
168
[wiki|RNA polymerase] beta' subunit
locus
BSU_01080
Molecular weight
133.92 kDa
pI
8.86
function
[wiki|transcription]
product
[wiki|RNA polymerase] beta' subunit
essential
yes
ec
2.7.7.6
synonyms
rpoC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0086 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
125,562 → 129,161
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
Nucleoside triphosphate + RNA(n) --> diphosphate + RNA(n + 1) (according to UniProt)
Protein family
[wiki|RNA polymerase] beta' chain family (single member, according to UniProt)
Structure
[PDB|3IYD] ([wiki|RNA polymerase] from ''E. coli'', 50% identity) [Pubmed|19903881]
[PDB|8EHQ] (the paused transcription complex of Mycobacterium tuberculosis [wiki|RNA polymerase] with B. subtilis NusG) [pubmed|36745813]
[PDB|8EOE] (the elongation complex of Mycobacterium tuberculosis [wiki|RNA polymerase] with B. subtilis NusG) [pubmed|36745813]
[AF|P37871]
Modification
phosphorylated on Arg-335 and Arg-803 [Pubmed|22517742]
[wiki|Localization]
colocalizes with the nucleoid [Pubmed|25092033]
Expression and Regulation
Operons
genes
[gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
description
[Pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7657605], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-30 13:43:49
Author of last update: Jstuelk