pupG
168
purine nucleoside phosphorylase
locus
BSU_23490
Molecular weight
28.98 kDa
pI
4.86
function
purine salvage and interconversion
product
purine nucleoside phosphorylase
essential
no
ec
2.4.2.1
synonyms
pupG, punA, pnp, yqkO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0005 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,446,418 → 2,447,233
The protein
Catalyzed reaction/ biological activity
purine 2'-deoxy-D-ribonucleoside + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + purine nucleobase (according to UniProt)
Protein family
PNP/MTAP phosphorylase family (single member, according to UniProt)
Structure
[PDB|3LA8] (from Streptococcus mutans, 57% identity)
[AF|P46354]
Modification
phosphorylation on Ser-28 [Pubmed|17218307]
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|9D07E43BF1FFB13CCEAC2B7AAA4EBDFB1079DD74|drm]-[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]
description
[Pubmed|10537218]
regulation
induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) [Pubmed|10537218]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10537218], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE23490 (Δ[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGG
BKK23490 (Δ[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGG
References
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Time of last update: 2024-12-29 20:01:44
Author of last update: Melvin.boenninger