treP
168
trehalose permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIBC of the [category|SW.1.2.2|PTS]
locus
BSU_07800
Molecular weight
49.84 kDa
pI
8.56
function
trehalose uptake and phosphorylation
product
trehalose-specific [category|SW.1.2.2|PTS] permease, EIIBC component
essential
no
ec
2.7.1.69
synonyms
treP, treB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
850,367 → 851,779
The protein
Catalyzed reaction/ biological activity
α,α-trehalose + Nπ-phospho-L-histidyl-[protein] --> α,α-trehalose 6-phosphate + L-histidyl-[protein] (according to UniProt)
Protein family
[category|SW.1.2.2|PTS] permease, sucrose family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIB domain] type-1 (aa 1-88) (according to UniProt)
[wiki|PTS EIIC domain] type-1 (aa 108-470) (according to UniProt)
Structure
[PDB|2IPJ] (from Clostridium difficile, the [wiki|PTS EIIB domain], 41.4% identity) [pubmed|17442338]
[AF|P39794]
Paralogous protein(s)
[protein|531F132F7F6A878F1E1D56977B9898A14272349A|sacX], [protein|AE02C38397AA790E4B216BB6DBABFE907B984D05|sacP], [protein|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP], [protein|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|BC568649A341B2E6341993EDA4BF52BDE18A3294|treP]-[gene|2EB9E0C492DF57DF683817E31D6DD34D7580E631|treA]-[gene|DC374E280C5A67C138D7EAC43AA66DA63CEF82B6|treR]
description
[Pubmed|8755887]
regulation
expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|21636651]
regulatory mechanism
[protein|DC374E280C5A67C138D7EAC43AA66DA63CEF82B6|treR]: repression, [Pubmed|8755887], in [regulon|protein:DC374E280C5A67C138D7EAC43AA66DA63CEF82B6|treR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|18977770], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|21636651], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8755887], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C259 (treP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2257 NBRP B. subtilis, Japan]
BKE07800 (Δ[gene|BC568649A341B2E6341993EDA4BF52BDE18A3294|treP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCAACCCCTCCGTATC, downstream forward: _UP4_TAACAAGTGGGGAGCGGGAC
BKK07800 (Δ[gene|BC568649A341B2E6341993EDA4BF52BDE18A3294|treP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCAACCCCTCCGTATC, downstream forward: _UP4_TAACAAGTGGGGAGCGGGAC
References
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Time of last update: 2024-12-21 23:29:25
Author of last update: Jstuelk