rapJ
168
response regulator aspartate phosphatase, dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-P
locus
BSU_02820
Molecular weight
44.25 kDa
pI
4.9
function
control of the [wiki|phosphorelay]
product
response regulator aspartate phosphatase
essential
no
synonyms
rapJ, ycdE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0457 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
304,430 → 305,551
The protein
Catalyzed reaction/ biological activity
dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-P [Pubmed|21346797]
Protein family
[wiki|RAP family] (according to UniProt)
[wiki|Domains]
RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain (five [wiki|TPR repeat|tetratrichopeptide repeats]) that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. [Pubmed|23526881]
Structure
[PDB|4GYO] (complex [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]-[protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]) [Pubmed|23526881]
[AF|O34327]
Effectors of protein activity
the interaction with [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC] inactivates [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ] [Pubmed|23526881]
Expression and Regulation
Operons
genes
[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]
description
[pubmed|22383849]
Biological materials
Mutant
BKE02820 (Δ[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT, downstream forward: _UP4_TAGGAAATGGCAGAGAACTA
BKK02820 (Δ[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT, downstream forward: _UP4_TAGGAAATGGCAGAGAACTA
References
Reviews
Original Publications
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Time of last update: 2024-12-21 13:49:52
Author of last update: Jstuelk