sdaAB
168
L-serine deaminase
locus
BSU_15850
Molecular weight
23.67 kDa
pI
5.1
function
serine utilization
product
L-serine deaminase (beta chain)
essential
no
ec
4.3.1.17
synonyms
sdaAB, yloW, sdaA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1760 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,658,242 → 1,658,904
Phenotypes of a mutant
overexpression of [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA] results in resistance to serine [pubmed|32743959]
The protein
Catalyzed reaction/ biological activity
L-serine --> NH4+ + pyruvate (according to UniProt)
Protein family
iron-sulfur dependent L-serine dehydratase family (with [protein|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA], according to UniProt)
[wiki|Domains]
[wiki|ACT domain] (aa 148-220) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[AF|O34635]
Expression and Regulation
Operons
genes
[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG
BKK15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG
lacZ fusion
pGP2275 (in [wiki|pAC5]) (GP2967), available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]
References
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Time of last update: 2024-12-25 08:53:10
Author of last update: Jstuelk