pdaB
168
polysaccharide deacetylase
locus
BSU_01570
Molecular weight
28.17 kDa
pI
9.18
function
spore cortex formation
product
polysaccharide deacetylase
essential
no
synonyms
pdaB, ybaN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
159,779 → 160,543
Phenotypes of a mutant
''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]'' and ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|D5B9ACB1E447A893C99A4ACE1DA95EB38BCF971B|ytrH]'' mutants are deficient in sporulation
inactivation of ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]'' reduces sporulation efficiency to 5% that of wild type cells [Pubmed|26735940]
The protein
Protein family
[wiki|polysaccharide deacetylase family] (according to UniProt)
[wiki|Domains]
[wiki|NodB homology domain] (aa 57-237) (according to UniProt)
Structure
[PDB|4M1B] (from B. anthracis, 54% identity) [pubmed|24637747]
[AF|P50865]
[wiki|Localization]
forespore (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]
description
[Pubmed|12662922]
regulation
expressed during sporulation in the mother cell [Pubmed|12662922]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|12662922], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
MGNA-B947 (ybaN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1946 NBRP B. subtilis, Japan]
BKE01570 (Δ[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGATGATGCCTCCTTAT, downstream forward: _UP4_GCCAAATCCGCAGAGGTAAA
BKK01570 (Δ[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGATGATGCCTCCTTAT, downstream forward: _UP4_GCCAAATCCGCAGAGGTAAA
References
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Time of last update: 2024-12-20 20:59:40
Author of last update: Melvin.boenninger