nrnB
168
sporulation-specific 3′-5′ exonuclease, degradation of pGpG
locus
BSU_18200
Molecular weight
46.28 kDa
pI
5.03
function
degradation of RNA oligonucleotides
product
sporulation-specific 3′-5′ exonuclease
essential
no
synonyms
nrnB, yngD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2404 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,948,313 → 1,949,512
The protein
Catalyzed reaction/ biological activity
degradation of RNA (both oligonucleotides and longer RNAs) (3′-5′ exonuclease activity) [Pubmed|37650646,19553197]
degradation of pGpG derived from c-di-GMP [pubmed|30249708]
Protein family
DHH/DHHA1 protein family of phosphoesterases [Pubmed|19553197]
[wiki|Domains]
[wiki|DHH-DHHA1 domain] [pubmed|36026528]
Structure
[AF|O31824]
Expression and Regulation
Operons
genes
[gene|BBB0848B2516188E950763E1D0194860923CE895|nrnB]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation] in the forespore [pubmed|37650646,22383849]
regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [pubmed|37650646], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [pubmed|37650646], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-B398 (yngD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1397 NBRP B. subtilis, Japan]
BKE18200 (Δ[gene|BBB0848B2516188E950763E1D0194860923CE895|nrnB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT, downstream forward: _UP4_TAAAAAAATATGCATAGGGG
BKK18200 (Δ[gene|BBB0848B2516188E950763E1D0194860923CE895|nrnB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT, downstream forward: _UP4_TAAAAAAATATGCATAGGGG
References
Reviews
Original Publications
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Time of last update: 2024-10-04 00:42:26
Author of last update: Jstuelk