araL
168
sugar phosphate phosphatase, L-arabinose operon
locus
BSU_28770
Molecular weight
29.16 kDa
pI
5.37
function
detoxification of accidental accumulation of phosphorylated metabolites
product
sugar phosphate phosphatase
essential
no
synonyms
araL, yseA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0647 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,944,187 → 2,945,005
The protein
Catalyzed reaction/ biological activity
H2O + O-phospho-L-serine --> L-serine + phosphate (according to UniProt)
H2O + O-phospho-D-serine --> D-serine + phosphate (according to UniProt)
Protein family
[wiki|HAD superfamily] (according to UniProt)
Structure
[PDB|3QGM] (from ''Archaeoglobus Fulgidus'' (35%) )
[AF|P94526]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]-[gene|E0525A4187D9C8A23F23BC8F883973896C8D295D|araB]-[gene|3859854178E573AE8ACCE6DF85E32C495D316DA1|araD]-[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]-[gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]-[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]-[gene|8AF43CA410F3381209196E1B9A4FE66D78020793|araP]-[gene|AE532A8931D516CC676E765D1D1293E73C335D23|araQ]-[gene|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA]
description
[Pubmed|9084180]
regulation
induced by arabinose ([protein|search|AraR]) [Pubmed|10417639]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12949161], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|A567466894AE9DE7CDE7816615433A37532297B5|araR]: repression, [Pubmed|10417639], in [regulon|protein:A567466894AE9DE7CDE7816615433A37532297B5|araR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9084180], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
MGNA-B015 (araL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1014 NBRP B. subtilis, Japan]
BKE28770 (Δ[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGCCAGCCGGTGACACAG, downstream forward: _UP4_TTGGCTGAGGAGGGGATTCT
BKK28770 (Δ[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGCCAGCCGGTGACACAG, downstream forward: _UP4_TTGGCTGAGGAGGGGATTCT
References
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Time of last update: 2024-10-07 12:25:26
Author of last update: Melvin.boenninger