rnc
168
RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
locus
BSU_15930
Molecular weight
28.28 kDa
pI
8.08
function
processing and degradation of RNA molecules
product
endoribonuclease III
essential
yes
synonyms
rnc, rncS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0571 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,665,710 → 1,666,459
Phenotypes of a mutant
essential [Pubmed|23300471,12682299,11123676]
the ''[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]'' gene can be deleted in strains cured of the [wiki|Skin element] and [wiki|SP-beta prophage] (or upon deletion of the toxin genes ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' and ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'') [Pubmed|23300471]
additional information
[wiki|RNase III targets| RNAs that are targeted by RNase III] [pubmed|26883633]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
cleaves 16S rRNA 2 nts downstream of the 3' end [PubMed|26883633]
cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]'')
degradation of ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' mRNA-[protein|search|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0] RNA duplex (encoded on the [wiki|Skin element]) [Pubmed|23300471]
degradation of ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'' mRNA-''[gene|8832F99BF927D5A4D6CB265DB24D3AE46434A5CD|SR6]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]
degradation of the ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]''-''[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]'' and ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]-[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]'' RNA duplexes [Pubmed|25643072]
Protein family
ribonuclease III family (single member, according to UniProt)
[wiki|Domains]
RNase III domain (aa 20-149) (according to UniProt)
DRBM domain (aa 175-222) (according to UniProt)
Structure
[PDB|1O0W] (from ''Thermotoga maritima'', 41% identity)
[AF|P51833]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]-[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]
description
[Pubmed|11021934]
regulation
expressed during vegetative growth and during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|11021934]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11021934], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Stülke] lab, [pubmed|19193632]
labs
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 09:20:36
Author of last update: Jstuelk