pghB
168
phage-derived gamma polyglutamate hydrolase
locus
BSU_12480
Molecular weight
22.70 kDa
pI
6.42
function
polyglutamate degradation
product
phage-derived gamma polyglutamic acid hydrolase
essential
no
synonyms
pghB, yjqB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4195 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,319,011 → 1,319,613
The protein
Catalyzed reaction/ biological activity
hydrolysis of polyglutamic acid [Pubmed|26158264]
Protein family
[wiki|UPF0714 family] (according to UniProt)
[wiki|Domains]
DUF867 [http://pfam.xfam.org/family/PF05908 x], [[gamma-PGA hydrolase domain]] [Pubmed|26158264]
Structure
[PDB|3A9L] (from B. subtilis phage NIT1, 41% identity) [pubmed|22105902]
[AF|O34785]
Paralogous protein(s)
[protein|DF95DED9A6E2160C151D7664C951B198EEA225E8|pghC], [protein|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL], [protein|8F9DCD77660706E4D25CD7875C014F6A2B5D8123|pghZ]
Expression and Regulation
Operons
genes
[gene|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB]-[gene|59EC45E8731FE989152553A69ACEF6569E88F85C|yjqC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B305 (yjqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1304 NBRP B. subtilis, Japan]
BKE12480 (Δ[gene|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAACGCCCCCCTTT, downstream forward: _UP4_TAAAACTAGACGGGACCTGC
BKK12480 (Δ[gene|BA7D1D112E934B68073DFC1F76248ED6157240E3|pghB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAACGCCCCCCTTT, downstream forward: _UP4_TAAAACTAGACGGGACCTGC
References
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Time of last update: 2025-03-13 03:22:29
Author of last update: Melvin.boenninger