ycbH
168
galactarate dehydratase
locus
BSU_02510
Molecular weight
54.63 kDa
pI
5.7
function
galactarate utilization
product
galactarate dehydratase
essential
no
ec
4.2.1.42
synonyms
ycbH, garD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2721 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
274,029 → 275,561
The protein
Catalyzed reaction/ biological activity
galactarate --> 5-dehydro-4-deoxy-D-glucarate + H2O (according to UniProt)
Protein family
uxaA family (with [protein|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA], according to UniProt)
Structure
[PDB|6U7L] (from E. coli, 67.6% identity)
[AF|P42240]
Expression and Regulation
Operons
genes
[gene|3542BC20C11985B3CFAA4F930E8D794700ADFD2E|ycbC]-[gene|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD]-[gene|9C4932DA4DEF99263E57871A99684E3DAD93E1EC|ycbE]-[gene|372F2A420309DB0193AEF36A740EBA26410F97F2|ycbF]-[gene|1A65880F68898002EE8774F34EDC47F0243B7273|ycbG]-[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]-[gene|349C99C258331962455100E2662DA2272E946C8A|ycbJ]
description
[Pubmed|12044674]
regulation
induced by D-glucarate/galactarate ([protein|search|YcbG]) [Pubmed|12044674]
regulatory mechanism
[protein|1A65880F68898002EE8774F34EDC47F0243B7273|ycbG]: repression, [Pubmed|12044674], in [regulon|protein:1A65880F68898002EE8774F34EDC47F0243B7273|ycbG regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18840696], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
Biological materials
Mutant
MGNA-C031 (ycbH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2029 NBRP B. subtilis, Japan]
BKE02510 (Δ[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAAAATCACTCCTCA, downstream forward: _UP4_TGATTCATGTTTTTATAGCG
BKK02510 (Δ[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAAAATCACTCCTCA, downstream forward: _UP4_TGATTCATGTTTTTATAGCG
References
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Time of last update: 2025-01-20 14:28:15
Author of last update: Jstuelk