yngJ
168
3-methylbutanoyl-CoA dehydrogenase
locus
BSU_18260
Molecular weight
40.76 kDa
pI
5.79
function
mother cell metabolism, leucine utilization
product
3-methylbutanoyl-CoA dehydrogenase
essential
no
ec
1.3.99.10
synonyms
yngJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1960 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,956,218 → 1,957,360
The protein
Catalyzed reaction/ biological activity
3-methylbutanoyl-CoA -→ 3-methylbut-2-enoyl-CoA [Pubmed|19935659]
A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH2 (according to UniProt)
Protein family
[wiki|Acyl-CoA dehydrogenase family] (according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|3OWA] (from ''B. anthracis'', 37% identity, 54% similarity)
[AF|O34421]
Paralogous protein(s)
[protein|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC], [protein|7D89BBB52403BF99416250688EFA90C2BE8EB591|acdA], [protein|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
Expression and Regulation
Operons
genes
[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
description
[Pubmed|12662922]
regulation
expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE18260 (Δ[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC, downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
BKK18260 (Δ[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC, downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
References
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Time of last update: 2024-12-20 15:07:44
Author of last update: Melvin.boenninger