narG
168
nitrate reductase (alpha subunit)
locus
BSU_37280
Molecular weight
138.86 kDa
pI
6.06
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (alpha subunit)
essential
no
synonyms
narG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5013 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,826,259 → 3,829,945
The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
Protein family
[wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
[wiki|Domains]
[wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 47-111) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [Pubmed|11289299]
MoCo [Pubmed|11289299]
Structure
[PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI] complex of E. coli, 52% identity) [pubmed|12910261]
[AF|P42175]
[wiki|Localization]
cell membrane [Pubmed|23880299]
Expression and Regulation
Operons
genes
[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
description
[Pubmed|8846791,16428414]
regulation
expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
regulatory mechanism
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
BKK37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
References
Reviews
Original Publications
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Time of last update: 2024-12-19 12:16:40
Author of last update: Jstuelk