rlmCD
168
rRNA methyltransferase
locus
BSU_06730
Molecular weight
51.65 kDa
pI
5.71
function
23S rRNA maturation
product
rRNA methyltransferase
essential
no
ec
2.1.1.190
synonyms
rlmCD, yerS, yefA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2265 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
737,603 → 738,982
The protein
Catalyzed reaction/ biological activity
methylation of U747 and U1939 in 23S rRNA [Pubmed|21824914]
S-adenosyl-L-methionine + uridine747 in 23S rRNA --> 5-methyluridine747 in 23S rRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
S-adenosyl-L-methionine + uridine1939 in 23S rRNA --> 5-methyluridine1939 in 23S rRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
[wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)
[wiki|Domains]
[wiki|TRAM domain] (aa 6-64) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|2BH2] (from ''E. coli'', 32% identity) [Pubmed|15766524]
[AF|O31503]
Paralogous protein(s)
[protein|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]
Expression and Regulation
Operons
genes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]
description
[Pubmed|22383849]
genes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A931 (yefA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/931 NBRP B. subtilis, Japan]
BKE06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
BKK06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
References
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Time of last update: 2024-12-21 17:22:18
Author of last update: Melvin.boenninger