hemAT
168
soluble chemotaxis receptor, heme-containing O2 sensor protein, senses ethanol and other short-chain alcohols
locus
BSU_10380
Molecular weight
48.60 kDa
pI
5.44
function
movement towards oxygen
product
haem-based aerotactic transducer
essential
no
synonyms
hemAT, yhfV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,112,620 → 1,113,918
Phenotypes of a mutant
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
NCIB3610 ''hemAT'' mutant has higher fitness than ''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mutant during pellicle formation [Pubmed|26122431]
NCIB3610 ''hemAT''-''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' double mutant has similar fitness to single ''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mutant during pellicle formation [Pubmed|26122431]
additional information
the gene readily acquires mutations during adaptation to growth as pellicle biofilm [pubmed|37768063]
The protein
Catalyzed reaction/ biological activity
required for full expression of the ''[gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]-[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[gene|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD]'' operon [Pubmed|23180473]
responsible for attractant response towards phenol and other aromatics at low (micromolar) concentrations [pubmed|35007157]
[wiki|Domains]
[wiki|Methyl-accepting transducer domain] (aa 184-420) (according to UniProt)
[wiki|Cofactors]
heme
Structure
[PDB|1OR4] (in cyano-liganded form), [PDB|1OR6]\t[Pubmed|12962628]
[AF|O07621]
[wiki|Localization]
forms clusters at the cell poles [Pubmed|21515776]
homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase [Pubmed|23180473]
polar foci in individual cells at later growth stages [Pubmed|23180473]
Expression and Regulation
Operons
genes
[gene|B948B9D96CDC43A07B3FF90212E52230A0EF1905|hemAT]
description
[Pubmed|15033535]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|15033535], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
additional information
in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell [PubMed|21515776]
Biological materials
Mutant
MGNA-B282 (yhfV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1281 NBRP B. subtilis, Japan]
TB239 (''hemAT''::''neo'' in 168) [Pubmed|26122431]
TB241 (''hemAT''::''neo'' in NCIB3610) [Pubmed|26122431]
BKE10380 (Δ[gene|B948B9D96CDC43A07B3FF90212E52230A0EF1905|hemAT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATACAATGATCCCCCTTG, downstream forward: _UP4_TAACCATCAAAAACCGGTCT
BKK10380 (Δ[gene|B948B9D96CDC43A07B3FF90212E52230A0EF1905|hemAT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATACAATGATCCCCCTTG, downstream forward: _UP4_TAACCATCAAAAACCGGTCT
labs
[wiki|Akos T Kovacs]
References
Reviews
Original Publications
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Time of last update: 2024-12-22 05:54:59
Author of last update: Jstuelk