rnhC
168
RNase HIII, endoribonuclease
locus
BSU_28620
Molecular weight
33.92 kDa
pI
10.07
function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules, processing of R-loops, maturation of Okazaki fragments
product
Mn2+-dependent RNase HIII
essential
no
ec
3.1.26.4
synonyms
rnhC, ysgB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1039 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,926,031 → 2,926,972
Phenotypes of a mutant
cold-sensisitive due to a defect in Okazaki fragment maturation [pubmed|30670546]
a [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC] double mutant is not viable [pubmed|33920686]
a [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC] [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] double mutant is not viable at 25°C [pubmed|30670546]
a [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] double mutant is not viable at 25°C [pubmed|30670546]
sensitive to hydroxyurea [pubmed|29084857]
a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]'' double mutant grows poorly [Pubmed|23882084]
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
maturation of Okazaki fragments (removal of RNA) (together with [protein|3D6DA8B52A4CB49361E67906140696FFA1EF6099|DNA polymerase I]) [pubmed|30670546]
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
Protein family
[wiki|RNase] HII family (with [protein|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB], according to UniProt)
[wiki|Cofactors]
Mn2+ [pubmed|29084857]
Structure
[PDB|2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity) [pubmed|16343535]
[PDB|2D0A] (Geobacillus stearothermophilus, 47% identity) [pubmed|16343535]
[AF|P94541]
[wiki|Localization]
recruited to the replication forks [pubmed|39195267]
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B002 (ysgB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1001 NBRP B. subtilis, Japan]
BP430 (''ΔrnhC::cat'') available in [wiki|Fabian Commichau]'s lab
BKE28620 (Δ[gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTAATAATCTCCTTTTT, downstream forward: _UP4_TAGAAAAAAGCTTGCAGATT
BKK28620 (Δ[gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTAATAATCTCCTTTTT, downstream forward: _UP4_TAGAAAAAAGCTTGCAGATT
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
Page visits: 3405
Time of last update: 2024-10-11 13:17:35
Author of last update: Jstuelk